Part:BBa_K3317018:Design

RBS designed for Vanillate O-demethylase (LigM gene) with sp

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

sequence optimized to e. coli BL21, the ecoRI, XbaI, SpeI and PstI sites were avoided, a signal peptide () was added at its C terminal end together with a flexible G4S linker

Source

ACCESSION BAK65949